GROWTH RATE AND BIOCHEMICAL CHARACTERIZATION OF CHLORELLA PYRENOIDOSA CULTIVATED IN SUGARCANE VINASSE MEDIUM

Chlorella pyrenoidosa is a microalgae species that contains proteins, carbohydrates, amino acids, carotenoids, vitamins, and minerals. Due to its compounds, the researchers have attempted make bioethanol using C. through biorefinery approach. However, ratio of production towards raw material needs still small because low carbohydrate content. Thus, in this research, vinasse used as growth medium increase The research objective study effect volume nutrient addition size, optical density, composition, rate pyrenoidosa, evaluation material. was cultivated freshwater (20 30% v/v) mini ponds, equipped with lighting 3280 lumens lamp, aeration air, Guillard nutrient. In vinasse, cultivation done without periodic additions. cell size increased if given addition, which 3.0-3.6 mm (in freshwater), 4.1-8.6 every 2 days), 4.8-6.3 days). composition sharply compared freshwater. very potential for production. Specific faster (0.087 day-1) than (0.023 (0.062 day-1). Cultivated lipids, so it has becoming